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Đánh giá mô hình gây suy thận mạn trên chuột nhắt trắng bằng Adenine
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Abstract
Objectives: This research aimed to evaluate the model of adenine - induced chronic kidney failure in white mice. Objects and research methods: 22 white mice were divided into 2 groups; The control group consisted of 10 healthy mice orally administered with distilled water. The experimental group was composed of 12 adenine - induced chronic kidney failure mice at a dosage of 100mg/kg/day, every 2 days for 5 weeks. Results: There is a statistically significant result in the experimental group, in which urea and creatinine concentrations were higher accompanied by the number red blood cells and the amount of hemoglobin, which were lower than that of the control group; Histopathology images showed the lesions in the glomerular and the interstitial proliferation. Overall survival rate of mice was 100%. Conclusions: the model of adenine - induced chronic kidney failure in white mice with adenine dose of 100mg/kg/day, every 2 days for 5 weeks showed typical symptoms and the proportion of mice still alive was 100%.
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Keywords
animal model, chronic kidney failure, adenine.
References
2. Diwan V, Brown L, Gobe GC (2018). "Adenine-induced chronic kidney disease in rats". Nephrology (Carlton, Vic), 23(1), 5-11.
3. Yokozawa T, Zheng PD, Oura
H, et al. (1986). "Animal model of adenine-induced chronic renal failure in rats". Nephron, 44(3), 230-4.
4. Jia T, Olauson H, Lindberg K, et al. (2013). "A novel model of adenine-induced tubulointerstitial nephropathy in mice". BMC nephrology, 14, 116.
5. Santana AC, Degaspari S, Catanozi S, et al. (2013). "Thalidomide suppresses inflammation in adenine-induced CKD with uraemia in mice". Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 28(5), 1140-9.
6. Al Suleimani YM, Al Za'abi M, Ramkumar A, et al. (2015). "Influence of treatment with gum acacia on renal vascular responses in a rat model of chronic kidney disease". European review for medical and pharmacological sciences, 19(3), 498-506.
7. Rahman A, Yamazaki D, Sufiun A, et al. (2018). "A novel approach to adenine-induced chronic kidney disease associated anemia in rodents". PloS one, 13(2), e0192531.
8. Phạm Xuân Phong, Lê Ngọc Thúy (2015). "Xây dựng mô hình suy thận mạn trên chuột nhắt". Tạp chí Y học Việt Nam, 430(2), 6-8.
9. Ali BH, Al-Salam S, Al Za'abi M, et al. (2013). "New model for adenine-induced chronic renal failure in mice, and the effect of gum acacia treatment thereon: comparison with rats". Journal of pharmacological and toxicological methods, 68(3), 384-93.
10. Yen CH, Sun CK, Leu S, et al. (2012). "Continuing exposure to low-dose nonylphenol aggravates adenine-induced chronic renal dysfunction and role of rosuvastatin therapy". Journal of translational medicine, 10, 147.